Radiosensitizing effects of curcumin alone or combined with GLUT1 siRNA on laryngeal carcinoma cells through AMPK pathway?induced autophagy

In this study, we investigated the ability of curcumin alone or in combination with GLUT1 siRNA to radiosensitize laryngeal carcinoma (LC) through induction autophagy. Protein levels tumour tissues and LC cells were measured by immunohistochemistry Western blotting. vitro, cell proliferation, colony formation assays, death autophagy detected. A nude mouse xenograft model was established injection Tu212 cells. We found that highly expressed negatively associated autophagy-related proteins suppressed radiation-mediated overexpression Treatment curcumin, siRNA, two promoted Inhibition using 6-amino-3-methypourine (3-MA) apoptosis after irradiation treatment siRNA. 3-MA inhibited siRNA-mediated non-apoptotic programmed death. The provided strongest sensitization vivo. also implicated AMP-activated protein kinase-mTOR-serine/threonine-protein kinase-Beclin1 signalling pathway. Irradiation primarily caused apoptosis, when combined treatment, increased radiosensitivity occurred concurrent